Characterization of esterase A , an autotransporter of Pseudomonas stutzeri A 15 1 2
نویسندگان
چکیده
* Corresponding author. Centre of Microbial and Plant Genetics, K.U. Leuven, Kasteelpark 11 Arenberg 20 – Bus 2460, 3001 Heverlee, Belgium. Tel.: +32 16321631. Fax: +32 16321963. E12 mail: [email protected] 13 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.07690-11 AEM Accepts, published online ahead of print on 3 February 2012
منابع مشابه
Characterization of Esterase A, a Pseudomonas stutzeri A15 Autotransporter.
Autotransporters are a widespread family of proteins, generally known as virulence factors produced by Gram-negative bacteria. In this study, the esterase A (EstA) autotransporter of the rice root-colonizing beneficial bacterium Pseudomonas stutzeri A15 was characterized. A multiple sequence alignment identified EstA as belonging to clade II of the GDSL esterase family. Autologous overexpressio...
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BACKGROUND Autotransporters represent a widespread family of secreted proteins in Gram-negative bacteria. Their seemingly easy secretion mechanism and modular structure make them interesting candidates for cell surface display of heterologous proteins. The most widely applied host organism for this purpose is Escherichia coli. Pseudomonas stutzeri A15 is an interesting candidate host for enviro...
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The search for new enzymes is facilitated by the rapidly growing number of genome sequences from different organisms. However, the discovery of functional proteins is still time intensive and complex alignments have to be performed. Herein, a genome database search identified a new, until now undescribed, putative lipase from Pseudomonas stutzeri strain A1501. The gene was cloned and expressed ...
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An estS9 gene, encoding an esterase of the psychrotolerant bacterium Pseudomonas sp. S9 was cloned and sequenced. The deduced sequence revealed a protein of 636 amino acid residues with a molecular mass of 69 kDa. Further amino acid sequence analysis revealed that the EstS9 enzyme contained a G-D-S-L motif centered at a catalytic serine, an N-terminal catalytic domain and a C-terminal autotrans...
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Background: Melanin is a negative charge hydrophobic complex pigment. Melanin is produced naturally in bacteria to protect them against UV, free radicals and environmental stresses. Pigment production in bacteria has more advantages than other biosources due to its rapid growth, higher efficiency and easier extraction. The aim of this study was the isolation, biochemical and molecular identific...
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تاریخ انتشار 2012